Twistnosticsllc LLC is a leading global provider of horseshoe crab blood and Limulus Amebocyte Lysate (LAL) products for endotoxin & β-glucan testing. We supply horseshoe crab blood for cGMP production and research purposes and manufacture LAL reagents for diagnostic endotoxin and β-glucan testing of products in the pharmaceutical and medical device industries. Choose from our selection of gel clot or quantitative assays, or let us manufacture custom reagents for your company or institution.

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Our horseshoe crab blood products play a vital role in ensuring the safety and efficacy of biotech products and medical treatments, including the upcoming COVID-19 vaccine. LAL has been and will remain the most trusted endotoxin testing reagent on the market.

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Twistnostics LLC specializes in global logistics solutions. We export blood and LAL reagents to countries in Europe, Asia, the Middle East, and more. To discuss a shipping solution…

The Horseshoe Crab and the Health of Humankind

If you’ve ever had to use contact lens solution, get immunization shots or common flu shots, their purity and your safety are ensured by the Limulus Polymephus Horseshoe crab. Vaccines, injectable drugs, intravenous solutions, and implantable medical devices, have to be quality checked utilizing LAL extracted from the blood of a Horseshoe crab.

How Does Horseshoe Crab Blood Help the Purity of Medicine?

People may become seriously ill if drugs aren’t purified of bacterial endotoxin or fungus (Beta 1~ 3 Glucans). It is especially dangerous if bacteria and its poisonous secretions known as (endotoxins) enter the human blood stream directly, such as from an injectable drug. Given this, drugs and implantable medical devices that come in contact with our blood stream are vulnerable and drugs going into our bodies must be tested to assure they do not contain any impurities. People with weakened immune systems are especially at risk (think of people undergoing chemotherapy, AIDS, or a person with diabetes) and impure medicines can be deadly to these individuals.The Horseshoe crab has an immune system that is elegantly simple at guarding itself from gram negative bacteria and its related poisons via bacteria secretion. Inside the Horseshoe crab’s circulatory (vascular) system, it contains only one cell and three blood proteins (that single cell in the blood is called the amebocyte). This cell contains proteins packed in tiny packets known a vesicles that are ready to be deployed if a foreign invader is found within the Horseshoe crab’s vascular (blood) stream. These proteins are released (degranulation) when the presence of foreign invaders like Gram negative bacteria are detected. This causes it’s blood to clot around the injury and bacteria, protecting the Horseshoe crab from infection.